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167 条 记 录,以下是 1-30

Response surface methodology for autolysis parameters optimization of shrimp head and amino acids released during autolysis
FOOD CHEMISTRYCao, Wenhong Zhang, Chaohua Hong, Pengzhi Ji, Hongwu  出版年:2008
Protein hydrolysates were prepared from the head waste of Penaens vannamei, a China seawater major shrimp by autolysis method. Autolysis conditions (viz., temperature, pH and substrate concentration) for preparing protei...
Autolysis of shrimp head by gradual temperature and nutritional quality of the resulting hydrolysate
LWT-FOOD SCIENCE AND TECHNOLOGYCao, Wenhong Zhang, Chaohua Hong, Pengzhi Ji, Hongwu Hao, Jiming Zhang, Jing  出版年:2009
Autolysis method was investigated for protein recovery from shrimp head (SH) of white shrimp (Penaeus vannamei). Autolysis of SH by several temperature conditions (40 degrees C, 50 degrees C, 60 degrees C and gradual tem...
Enzymatic hydrolysis optimization of Paphia undulata and lymphocyte proliferation activity of the isolated peptide fractions
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTUREHe, Xiao Qing Cao, Wen Hong Pan, Guang Kun Yang, Liu Zhang, Chao Hua  出版年:2015
BACKGROUNDThe immune system is important in preventing and controlling various infectious diseases. Immunoactive peptides derived from food proteins could exert immunomodulatory activity without side effects. Paphia undu...
Purification and identification of an ACE inhibitory peptide from the peptic hydrolysate of Acetes chinensis and its antihypertensive effects in spontaneously hypertensive rats
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGYCao, Wenhong Zhang, Chaohua Hong, Pengzhi Ji, Hongwu Hao, Jiming  出版年:2010
P>Acetes chinensis is a marine shrimp found in the coastal waters of China. The shrimp was hydrolysed by pepsin to prepare hydrolysates with angiotensin I-converting enzyme (ACE) inhibitory activity. The hydrolysate with...
Inactivation of polyphenol oxidase from Pacific white shrimp by dense phase carbon dioxide
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIESZhang, Liang Liu, Shucheng Ji, Hongwu Zhang, Chaohua Deng, Chujin Cao, Wenhong Mao, Weijie Gao, Jialong  出版年:2011
The inactivation of polyphenol oxidase (PPO) from Pacific white shrimp exposed to dense phase carbon dioxide (DPCD) treatment was investigated. PPO activity showed a dramatic loss at 4-25 MPa and 37 degrees C. At the low...
Antioxidant activity of bigeye tuna (Thunnus obesus) head protein hydrolysate prepared with Alcalase
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGYYang, Ping Ke, Hongqiao Hong, Pengzhi Zeng, Shaokui Cao, Wenhong  出版年:2011
The antioxidant activities of tuna head protein hydrolysate (THPH) prepared with Alcalase were evaluated. THPH showed evident radical scavenging activity in a dose-dependent manner with the IC50 values for 1,1-diphenyl-2...
牡蛎酶解产物的组成特点及其体外免疫活性
《食品工业科技》李婉 曹文红 章超桦 秦小明 高加龙 郑慧娜  出版年:2017
香港牡蛎是我国南方高产低值的一种海洋贝类。为了提高其附加值,本研究以香港牡蛎为原料,通过酶法水解结合细胞实验对酶解产物及其超滤组分进行组成特点分析和免疫活性评价。结果表明:牡蛎酶解产物及各超滤组分分子量分布主要集中在<2...
关键词:牡蛎解产物  脾淋巴细胞  腹腔巨噬细胞  免疫调节  被引量:23
Optimization of peptic hydrolysis parameters for the production of angiotensin I-converting enzyme inhibitory hydrolysate from Acetes chinensis through Plackett-Burman and response surface methodological approaches
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURECao, Wenhong Zhang, Chaohua Ji, Hongwu Hao, Jiming  出版年:2012
BACKGROUND: Angiotensin I-converting enzyme (ACE) plays an important physiological role in regulating blood pressure. The elevation of blood pressure could be suppressed by inhibiting ACE. ACE inhibitory peptides derived...
Preparation and characterisation of the pearl oyster (Pinctada martensii) meat protein hydrolysates with a high Fischer ratio
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGYZheng, Huina Zhang, Chaohua Cao, Wenghong Liu, Shucheng Ji, Hongwu  出版年:2009
The primary aim of this study was to obtain high Fischer ratio oligopeptides from the pearl oyster Pinctada martensii meat via proteolysis. Pinctada martensii meat protein was hydrolysed using seven proteases, with pancr...
Angiotensin I-converting enzyme inhibitory activity of Acetes chinensis peptic hydrolysate and its antihypertensive effect in spontaneously hypertensive rats
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGYZhang, Chaohua Cao, Wenhong Hong, Pengzhi Ji, Hongwu Qin, Xiaoming He, Jinfeng  出版年:2009
P>Peptic hydrolysate with angiotensin I-converting enzyme (ACE) inhibitory activity was prepared from Acetes chinensis. The 3 kDa ultrafiltration filtrates (UF-IV) of the desalted hydrolysate exerted a potent ACE inhibit...
牡蛎蛋白酶解物的制备及其抗氧化活性研究
《食品工业科技》林海生 曹文红 章超桦 黄嘉莉  出版年:2013
选用中性蛋白酶,研究了前处理方法、E/S、pH、温度和时间对酶解牡蛎蛋白效果的影响,制备牡蛎蛋白酶解物,分析其游离氨基酸含量及分子量分布,体外实验测定其抗氧化活性。结果表明,酶解前100℃热处理10min能够提高酶解物中...
关键词:前处理  牡蛎  酶解  抗氧化活性  被引量:18
冷冻方式对凡纳滨对虾品质特性的影响
《食品与机械》杨利艳 曹文红 章超桦 吉宏武  出版年:2011
以质构、盐溶性蛋白、ATPase活性、巯基含量、失水率及pH为指标,研究液氮速冻、-75℃超低温速冻和-18℃冷库冷冻3种冷冻方式对凡纳滨对虾品质特性的影响。结果表明:液氮速冻处理组失水率最低,盐溶性蛋白含量、巯基含量、...
关键词:凡纳滨对虾  液氮速冻  超低温速冻  品质特性  被引量:17
Optimisation of extraction conditions and characteristics of skin gelatin from Nile tilapia (Oreochromis niloticus)
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGYZeng, Shaokui Yan, Xiaoyan Cao, Wenhong Hong, Pengzhi Zhang, Chaohua Li, Laihao  出版年:2010
P>Response surface methodology was used to optimise gelatin extraction conditions from the skin of Nile tilapia (Oreochromis niloticus), and characteristics of the gelatin were determined. Concentration of NaOH (%, X-1),...
Ultraviolet irradiation and gradient temperature assisted autolysis for protein recovery from shrimp head waste
FOOD CHEMISTRYCao, Wenhong Tan, Caiyun Zhan, Xiaojian Li, Huiyi Zhang, Chaohua  出版年:2014
A novel autolysis method using ultraviolet (UV) irradiation and gradient temperature was investigated to efficiently recover proteins from the head of the shrimp Penaeus vannamei. The proteolytic activity of shrimp head ...
响应面法优化南美白对虾虾头自溶工艺的研究
《中国食品学报》曹文红 章超桦 洪鹏志 吉宏武  出版年:2009
目的:分析南美白对虾虾头的一般营养成分,利用自溶作用回收南美白对虾虾头中的蛋白质等营养成分,对自溶工艺参数进行优化。方法:采用AOAC(1990)推荐的常规成分分析法分析虾头的基本营养成分,通过响应面法的中心组合设计优化...
关键词:南美白对虾  虾头  自溶  响应面法  水解度  被引量:16
企鹅珍珠贝肉酶解产物的制备及其醒酒作用的初步研究
《食品工业科技》左光扬 章超桦 高加龙 秦小明 曹文红  出版年:2012
以企鹅珍珠贝肉为研究对象,通过不同种类的蛋白酶对其进行水解,获得具有醒酒效果的酶解产物。通过比较不同酶解产物水解度、蛋白回收率及其与酶解产物灌胃小鼠后血液中酒精浓度的相互关系,选择具有显著效果的酶解产物并对其主要成分进行...
关键词:企鹅珍珠贝  酶解  醒酒  血液中酒精浓度  被引量:16
Optimising the free radical scavenging activity of shrimp protein hydrolysate produced with alcalase using response surface methodology
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGYCao, Wenhong Zhang, Chaohua Hong, Pengzhi Ji, Hongwu  出版年:2009
P>Response surface methodology (RSM) was used to optimise the hydrolysis parameters of Acetes chinensis by Alcalase 2.4L in order to obtain a hydrolysate with potent radical scavenging activity. The parameters were tempe...
海湾扇贝酶解产物清除自由基活性的研究
《食品与发酵工业》张一江 曹文红 毕春波  出版年:2008
利用双酶(胰酶0.3%和枯草杆菌中性蛋白酶0.8%)。在50℃、pH值7.0~8.0、料水比1:3的条件下,对海湾扇贝肉酶解4h后制备得到产物.该酶解产物对羟自由基的清除活性IC_(50)为2.01 mg/mL,对超氧阴...
关键词:海湾扇贝  酶解  自由基  清除  分子质量分布  被引量:15
Novel insight into the role of processing stages in nutritional components changes and characteristic flavors formation of noble scallop Chlamys nobilis adductors
FOOD CHEMISTRYChen, Zhongqin Zhu, Yahui Cao, Wenhong Zhou, Longjian Zhang, Chaohua Qin, Xiaoming Zheng, Huina Lin, Haisheng Gao, Jialong  出版年:2022
Processing stages play critical role in the nutrition and flavor changes of marine products. This study investigated the nutrition and flavor profiles in noble scallop Chlamys nobilis adductor during boiling, rinsing, ba...
酶解中国毛虾制备低分子肽的研究
《食品与发酵工业》曹文红 章超桦 秦小明  出版年:2006
以中国毛虾为原料,采用酶法制备低分子肽。利用响应面法优化了Alcalase4L酶水解中国毛虾的工艺参数:温度57.1℃、pH8.0、加酶量为46mL/kg,酶解产物的平均肽链长从0.5h的14.02逐渐降低到8h的3.9...
关键词:中国毛虾  酶解  水解度  低分子肽  中心组合设计  被引量:14
波纹巴非蛤蛋白酶解产物的抗氧活性及分子量分布研究
《现代食品科技》何小庆 曹文红 章超桦 赵子科  出版年:2014
以波纹巴非蛤为原料,分离得到肌浆蛋白、肌原纤维蛋白、基质蛋白3种蛋白组分,分别研究其氨基酸组成及其胰蛋白酶酶解产物的体外抗氧化活性及分子量分布。结果表明该三种蛋白质中必需氨基酸、碱性氨基酸、疏水性氨基酸和支链氨基酸含量较...
关键词:波纹巴非蛤  氨基酸  抗氧化活性  分子量分布  被引量:14
中国毛虾酶解产物的分子量分布及ACE抑制活性
《食品科技》曹文红 秦小明 章超桦 王长云  出版年:2007
利用胃蛋白酶酶解中国毛虾制备具有血管紧张素转化酶抑制活性的酶解产物,并采用凝胶色谱法探讨酶解产物的分子量分布。结果表明:在45℃、pH2.5、酶量2%(w/w)、酶解4h、底物浓度20%(w/v)条件下,中国毛虾酶解产物...
关键词:中国毛虾  酶解  ACE抑制活性  分子量分布  被引量:12
南海不同产地近江牡蛎中牛磺酸含量检测
《食品科学》高加龙 章超桦 邱伟佳 曹文红 秦小明  出版年:2013
建立牡蛎中牛磺酸含量检测的高效液相色谱方法。近江牡蛎样品经超声波处理,所得样液采用丹磺酰氯柱前衍生后C18色谱柱分离,紫外检测器定量检测,方法检出限1.2×10-5μg/mL,牛磺酸质量浓度在0.2~2.2μg/L范围内...
关键词:近江牡蛎  牛磺酸  高效液相色谱  衍生  被引量:11
超滤法浓缩富集牡蛎蛋白抗氧化活性肽
《食品工业科技》郝更新 曹文红 郝记明 章超桦  出版年:2013
为研究牡蛎蛋白酶解产物中发挥抗氧化活性的肽片段,采用超滤的方法对其进行初步分离富集,测定了各级组分的体外抗氧化能力和氨基酸组成。结果表明:<4000u超滤液对羟自由基、DPPH自由基和超氧自由基清除活性的IC50值分别为...
关键词:牡蛎蛋白  酶解产物  超滤  抗氧化活性  被引量:11
华贵栉孔扇贝酶法制备α-葡萄糖苷酶抑制肽工艺优化
《广东海洋大学学报》林海生 廖津 章超桦 秦小明 曹文红 高加龙 罗凯耀  出版年:2020
【目的】为了进一步挖掘海洋贝类潜在的降血糖功能。【方法】选用华贵栉孔扇贝闭壳肌为原料,以抑制α-葡萄糖苷酶活性为评价指标,采用正交试验法和响应面分析法优化α-葡萄糖苷酶抑制肽的制备工艺。【结果】华贵栉孔扇贝(Chlamy...
关键词:酶解  生物活性肽  α-葡萄糖苷酶抑制率  响应面法  被引量:11
不同温度无水保活对香港牡蛎微生物和基本营养成分的影响
《广东海洋大学学报》高加龙 章超桦 秦小明 曹文红 郑惠娜 林海生  出版年:2020
【目的】研究温度对无水保活香港牡蛎(Crassostrea hongkongensis)存活状态、微生物以及营养品质的影响。【方法】对在4℃、10℃和15℃条件下保活不同时间的牡蛎存活率、菌落总数和大肠菌群数以及主要营养...
关键词:香港牡蛎  无水保活  微生物  营养成分  被引量:10
马氏珠母贝酶解产物清除自由基活性的研究
《食品研究与开发》曹文红 吴红棉 范秀萍 杜志伟 冯淑冰  出版年:2009
利用胰酶、枯草杆菌中性蛋白酶,在50℃、pH为7.0~8.0、料水比1:3的条件下,对马氏珠母贝肉双酶水解4h制备得到酶解产物及残渣。利用Alcalase2.4L、胃蛋白酶、复合蛋白酶、木瓜蛋白酶对残渣进行再次酶解。结果...
关键词:马氏珠母贝  酶解  自由基清除活性  分子量分布  被引量:10
虾头内源酶酶解物的ACE抑制活性分析
《食品与发酵工业》郝更新 章超桦 曹文红  出版年:2007
采用正交实验对影响虾头自溶的因素(pH值、温度、固液比和自溶时间)进行了优化,优化条件为:45℃,pH4,固液比1:3,时间2h,其ACE抑制率达到了40.7%(2mg/mL)。分析测定了经Sephadex G-25分离...
关键词:虾头  内源酶  酶解  分离  氨基酸组成  被引量:10
In Vitro Antioxidant Activity and In Vivo Anti-fatigue Effects of Oyster (Ostrea plicatula Gmelin) Peptides Prepared Using Neutral Proteinase
FOOD SCIENCE AND TECHNOLOGY RESEARCHHao, Gengxin Cao, Wenhong Hao, Jiming Zhang, Chaohua  出版年:2013
The amino acid profile of oyster (Ostrea plicatula Gmelin) peptides (OP) was assayed. Its in vitro antioxidant activity was determined, and its in vivo anti-fatigue activity was compared with those of oyster meat and oys...
食品蛋白来源生物活性肽
《粮食与油脂》曹文红 章超桦 秦小明  出版年:2007
生物活性肽是蛋白质功能片段经酶解产生具有特殊生理调节功能肽段。该文主要介绍降血压肽、高F值寡肽、酪蛋白磷酸肽、免疫活性肽、清除自由基活性肽等几种国内外广泛关注生物活性肽,并在此基础上对生物活性肽开发进行展望。
关键词:食品蛋白质  生物活性肽  酶解  被引量:9
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